Sugita T, Shiraki K, Ueda S, Iwa N, Shoji H, Ayata M, Kato S
J Infect Dis. 1984 Sep;150(3):340-7. doi: 10.1093/infdis/150.3.340.
Myoclonus is a characteristic neurological sign of subacute sclerosing panencephalitis (SSPE). Attempts were made to induce myoclonus in a large proportion of hamsters with a cell-associated strain of SSPE virus (the Biken strain) and thereby to establish an experimental model for study of the mechanism of development of this condition. When injected intracerebrally, Biken virus induced myoclonus within two to 14 days in 84% of the three- to nine-week-old hamsters tested. Electroencephalographic traces showed a periodic and synchronous discharge consisting of high-voltage slow waves and spikes that appeared coincidentally with myoclonus. Neurons in the cortex and thalamus of the affected animals had severely degenerated cytoplasm. Inflammatory changes, such as perivascular cuffing or infiltration of mononuclear cells, were not detected. Staining with immunoperoxidase revealed measles viral antigens in the cytoplasm and dendrites of the affected neurons. SSPE virus with the same properties as the parent virus was recovered from brain cells of sick animals by cocultivation with Vero cells.
肌阵挛是亚急性硬化性全脑炎（SSPE）的特征性神经学体征。人们试图用细胞相关的SSPE病毒株（比肯株）在大部分仓鼠中诱发肌阵挛，从而建立一个用于研究该病发病机制的实验模型。当脑内注射时，比肯病毒在2至14天内使84%接受测试的3至9周龄仓鼠诱发了肌阵挛。脑电图显示有周期性同步放电，由高压慢波和棘波组成，与肌阵挛同时出现。患病动物大脑皮质和丘脑的神经元胞质严重退化。未检测到炎症变化，如血管周围套袖现象或单核细胞浸润。免疫过氧化物酶染色显示，在受影响神经元的胞质和树突中有麻疹病毒抗原。通过与非洲绿猴肾细胞（Vero细胞）共培养，从患病动物的脑细胞中分离出了与亲本病毒特性相同的SSPE病毒。
