Sabinski F, Wosiewitz U
J Clin Chem Clin Biochem. 1984 Jul;22(7):453-9. doi: 10.1515/cclm.1984.22.7.453.
Glycoproteins were investigated in different types of biliary calculi by a methanolysis procedure of the carbohydrate moiety, the formation of 2,6-dinitro-4-trifluoromethylbenzenesulphonate derivatives of N-acetylhexosamines and N-acetylneuraminic acid, followed by separation, identification and quantification of the liberated monosaccharides using capillary gas-liquid chromatography. This indirect method avoids several analytical problems caused by the presence of bile pigment derivatives; unless these are carefully removed they interfere strongly in the measurement of protein. The carbohydrate contents were much higher in pigment stones than in cholesterol and mixed stones. Rhamnose, a non-mammalian monosaccharide, was identified in most samples, but was significant increased in pigment stones, indicating a bacterial history of this stone type.
通过对碳水化合物部分进行甲醇解、将N-乙酰己糖胺和N-乙酰神经氨酸转化为2,6-二硝基-4-三氟甲基苯磺酸酯衍生物，随后使用毛细管气液色谱法对释放的单糖进行分离、鉴定和定量，对不同类型的胆结石中的糖蛋白进行了研究。这种间接方法避免了由胆汁色素衍生物的存在引起的几个分析问题；除非仔细去除这些衍生物，否则它们会强烈干扰蛋白质的测量。色素结石中的碳水化合物含量远高于胆固醇结石和混合结石。鼠李糖，一种非哺乳动物单糖，在大多数样本中都被鉴定出来，但在色素结石中显著增加，表明这种结石类型有细菌感染史。
