Voskresenskaya E, Savin C, Leclercq A, Tseneva G, Carniel E
Yersinia Research Unit, National Reference Laboratory, WHO Collaborating Center for Yersinia, Institut Pasteur, Paris, France Laboratory of Bacterial Respiratory Infections, Institut Pasteur, Saint Petersburg, Russia.
Yersinia Research Unit, National Reference Laboratory, WHO Collaborating Center for Yersinia, Institut Pasteur, Paris, France.
J Clin Microbiol. 2014 Jun;52(6):1978-89. doi: 10.1128/JCM.00397-14. Epub 2014 Mar 26.
Yersinia pseudotuberculosis is an enteropathogen that has an animal reservoir and causes human infections, mostly in temperate and cold countries. Most of the methods previously used to subdivide Y. pseudotuberculosis were performed on small numbers of isolates from a specific geographical area. One aim of this study was to evaluate the typing efficiency of restriction fragment length polymorphism of insertion sequence hybridization patterns (IS-RFLP) compared to other typing methods, such as serotyping, ribotyping, and multilocus sequence typing (MLST), on the same set of 80 strains of Y. pseudotuberculosis of global origin. We found that IS100 was not adequate for IS-RFLP but that both IS285 and IS1541 efficiently subtyped Y. pseudotuberculosis. The discriminatory index (DI) of IS1541-RFLP (0.980) was superior to those of IS285-RFLP (0.939), ribotyping (0.944), MLST (0.861), and serotyping (0.857). The combination of the two IS (2IS-RFLP) further increased the DI to 0.998. Thus, IS-RFLP is a powerful tool for the molecular typing of Y. pseudotuberculosis and has the advantage of exhibiting well-resolved banding patterns that allow for a reliable comparison of strains of worldwide origin. The other aim of this study was to assess the clustering power of IS-RFLP. We found that 2IS-RFLP had a remarkable capacity to group strains with similar genotypic and phenotypic markers, thus identifying robust populations within Y. pseudotuberculosis. Our study thus demonstrates that 2IS- and even IS1541-RFLP alone might be valuable tools for the molecular typing of global isolates of Y. pseudotuberculosis and for the analysis of the population structure of this species.
假结核耶尔森菌是一种肠道病原体，它以动物为储存宿主并可导致人类感染，主要发生在温带和寒冷国家。以前用于细分假结核耶尔森菌的大多数方法都是对来自特定地理区域的少量分离株进行的。本研究的一个目的是，在同一组80株全球来源的假结核耶尔森菌上，评估插入序列杂交模式的限制性片段长度多态性（IS-RFLP）与其他分型方法（如血清分型、核糖体分型和多位点序列分型（MLST））相比的分型效率。我们发现IS100不适用于IS-RFLP，但IS285和IS1541都能有效地对假结核耶尔森菌进行亚型分型。IS1541-RFLP的鉴别指数（DI）（0.980）优于IS285-RFLP（0.939）、核糖体分型（0.944）、MLST（0.861）和血清分型（0.857）。两种IS的组合（2IS-RFLP）进一步将DI提高到0.998。因此，IS-RFLP是假结核耶尔森菌分子分型中的一种强大工具，其优势在于能呈现出分辨率良好的条带模式，从而可以对全球来源的菌株进行可靠比较。本研究的另一个目的是评估IS-RFLP的聚类能力。我们发现2IS-RFLP具有显著的能力，能够将具有相似基因型和表型标记的菌株聚集在一起，从而在假结核耶尔森菌中识别出稳定的群体。因此，我们的研究表明，单独的2IS-甚至IS1541-RFLP可能是对全球假结核耶尔森菌分离株进行分子分型以及分析该物种群体结构的有价值工具。
