Sulpice J C, Férézou J
Lipids. 1984 Aug;19(8):631-5. doi: 10.1007/BF02534723.
A new procedure is described for isolating and measuring squalene in plasma and in several organs of the rat. The unsaponifiable material was fractionated by normal phase HPLC on a silica gel column using a mobile phase consisting of hexane/propanol-2/water. The eluate was monitored at 215 nm. The squalene in the hydrocarbon fraction thus collected was than quantified on an analytical column eluted with hexane. Squalene concentrations ranging from 3 to 200 micrograms per ml of plasma or per g of fresh tissue were accurately measured. The results obtained agree with those of the squalene assays carried out by gas chromatography on a packed or capillary column.
本文描述了一种用于分离和测定大鼠血浆及多个器官中角鲨烯的新方法。不皂化物通过正相高效液相色谱法在硅胶柱上进行分离，流动相为己烷/2-丙醇/水。在215nm处监测洗脱液。然后，将如此收集的烃类馏分中的角鲨烯在以己烷洗脱的分析柱上进行定量。每毫升血浆或每克新鲜组织中角鲨烯浓度在3至200微克范围内均可准确测定。所得结果与在填充柱或毛细管柱上通过气相色谱法进行的角鲨烯测定结果一致。
