Aitzetmüller K, Koch J
J Chromatogr. 1978 Mar 1;145(2):195-202. doi: 10.1016/s0378-4347(00)81339-0.
A technique is described for the high-pressure liquid chromatographic (HPLC) analysis of sebum lipid classes. The lipid present in sebum are separated by gradient elution HPLC from a microparticulate silica column and detected using a moving-wire detector. The system described can be linked to a computer. Quantitation can be carried out by comparing peak areas obtained with those of an internal standard. Peak trapping for further investigations of the separated components, for example by gas chromatography-mass spectrometry, is very easy. Sebum lipids are separated into the following lipid classes: hydrocarbons and squalene, cholesterol esters and wax esters, fatty acids as their methyl esters, triglycerides, 1,3-diglycerides, 1,2-diglycerides, free cholesterol, monoglycerides and other polar materials. Besides to sebum, the method has been successfully applied to other lipid mixtures, such as serum lipids. Examples of other applications are shown.
本文描述了一种用于皮脂脂质类别的高压液相色谱（HPLC）分析技术。皮脂中的脂质通过梯度洗脱HPLC从微粒硅胶柱上分离出来，并使用移动丝检测器进行检测。所描述的系统可以连接到计算机。通过将获得的峰面积与内标峰面积进行比较来进行定量。对于分离出的组分进行进一步研究（例如通过气相色谱 - 质谱法）的峰捕集非常容易。皮脂脂质可分为以下脂质类别：烃类和角鲨烯、胆固醇酯和蜡酯、作为甲酯的脂肪酸、甘油三酯、1,3 - 二甘油酯、1,2 - 二甘油酯、游离胆固醇、单甘油酯和其他极性物质。除了皮脂外，该方法已成功应用于其他脂质混合物，如血清脂质。还展示了其他应用实例。
