Bern H A, Mills K T, Mori T
Proc Soc Exp Biol Med. 1984 Nov;177(2):303-7. doi: 10.3181/00379727-177-41947.
In two experiments, neonatal female BALB/cCrgl or BALB/cfC3HCrgl mice were given subcutaneous injections of 5 micrograms 17 beta-estradiol or sesame oil for the first 3 days of life and were ovariectomized at 60 days of age, at which time vaginal concretions (Experiments I and II) or silica (Experiment II) were implanted intravaginally. Mice were examined at 12 months of age. Three abnormal cervicovaginal epithelial responses were noted: persistent vaginal stratification/cornification (PVS); prominent vaginal squamocolumnar junction (SCJ); epithelial pegs, downgrowths, or lesions (dysplasias). PVS, not present in unimplanted controls, occurs in at least half of the members of the neonatally estrogen-treated groups; implants of concretions or silica did not increase its incidence significantly. Although SCJ was observed in implanted but not in unimplanted controls, its incidence was significantly higher in neonatally estrogen-treated mice than in either control group. The elevated incidence in neonatally estrogen-treated mice was not increased further by implantation of concretions or silica. In neonatally estrogenized mice, the subsequent implantation of a concretion significantly increased the incidence of cervicovaginal abnormalities. Increased PVS and SCJ are teratological consequences of neonatal exposure to a small amount of estrogen; on the other hand, increased dysplasias may, in part, be responses of the estrogenized vaginal epithelium to the concretions.
在两项实验中，新生雌性BALB/cCrgl或BALB/cfC3HCrgl小鼠在出生后的头3天皮下注射5微克17β-雌二醇或芝麻油，并在60日龄时进行卵巢切除，此时将阴道结石（实验I和II）或二氧化硅（实验II）经阴道植入。在12月龄时对小鼠进行检查。观察到三种异常的宫颈阴道上皮反应：持续性阴道分层/角化（PVS）；明显的阴道鳞柱交界（SCJ）；上皮钉突、向下生长或病变（发育异常）。PVS在未植入的对照组中不存在，在新生期接受雌激素处理的组中至少一半的小鼠出现；植入结石或二氧化硅并未显著增加其发生率。虽然在植入组而非未植入组中观察到了SCJ，但其在新生期接受雌激素处理的小鼠中的发生率显著高于任何一个对照组。新生期接受雌激素处理的小鼠中SCJ发生率的升高并未因植入结石或二氧化硅而进一步增加。在新生期经雌激素处理的小鼠中，随后植入结石显著增加了宫颈阴道异常的发生率。PVS和SCJ的增加是新生期暴露于少量雌激素的致畸后果；另一方面，发育异常的增加可能部分是经雌激素处理的阴道上皮对结石的反应。
