Frasca P, Buchanan J W, Soriano R Z, Dunn J M, Marmon L, Melbin J, Buchanan S J, Chang S H, Golub E E, Shapiro I M
Scan Electron Microsc. 1984(Pt 2):973-7.
Glutaraldehyde fixed patch grafts of bovine pericardium were implanted in myocardial windows in young (3-4 months old) sheep. The samples were retrieved after one to three weeks for study with scanning electron microscopy (SEM) and energy dispersive x-ray microanalysis (EDX). A layer of porous material (pseudoneointima, PNI), consisting mostly of a dense mesh of fibers interspersed with blood cells, was noted to form on the blood contacting surface of the graft. Four distinct sets of mineralization were noted in the retrieved grafts: (1) at the blood contacting surface of the PNI; (2) within the PNI at the junction between layers of PNI with differing densities; (3) near the junction of PNI and pericardium (but in the PNI); and (4) within the pericardium. In both the PNI and pericardium the mineral was shown by EDX analysis to contain both calcium and phosphorous indicating the mineral to be a calcium phosphate. Mineralization in the PNI differed from that in the pericardium; in the PNI it was deposited in discrete regions and apparently in association with thrombi while in the pericardium it was distributed diffusely within the collagen matrix, which may influence its formation.
将戊二醛固定的牛心包补片植入年轻（3 - 4个月大）绵羊的心肌窗口。一至三周后取出样本，用于扫描电子显微镜（SEM）和能量色散X射线微分析（EDX）研究。在移植物与血液接触的表面形成了一层多孔材料（假内膜，PNI），主要由密集的纤维网和散在的血细胞组成。在取出的移植物中发现了四组不同的矿化：（1）在PNI与血液接触的表面；（2）在PNI内不同密度层之间的交界处；（3）在PNI与心包交界处附近（但在PNI内）；（4）在心包内。通过EDX分析表明，PNI和心包中的矿物质都含有钙和磷，表明该矿物质是磷酸钙。PNI中的矿化与心包中的不同；在PNI中，矿物质沉积在离散区域，显然与血栓有关，而在心包中，矿物质分散分布在胶原基质中，这可能会影响其形成。
