α2-抗纤溶酶的两种形式：KAV和等电点的差异。

Two forms of alpha 2-antiplasmin: differences in KAV and isoelectric point.

作者信息

Högstorp H, Jacobsson H, Wallin R, Saldeen T

出版信息

Thromb Res. 1984 Sep 1;35(5):559-65. doi: 10.1016/0049-3848(84)90287-1.

Abstract

The plasminogen-binding (PB-AP) and the non-plasminogen-binding (NPB-AP) forms of alpha 2-antiplasmin were assayed by a modified rocket electroimmunoassay technique using an intermediary gel containing kringles 1-3 from plasminogen. Gel chromatography of normal human plasma (HNP) showed differences in KAV between PB-AP and NPB-AP. PB-AP had a KAV of 0.33 and NPB-AP a KAV of 0.39. Isoelectric focusing of HNP showed pI differences between PB-AP and NPB-AP. PB-AP was heterogeneous, with a main peak at pI = 4.69 and a minor peak at pI = 4.92, while NPB-AP had only one peak, at pI = 4.59.

摘要

使用含有纤溶酶原kringles 1-3的中间凝胶，通过改良的火箭免疫电泳技术检测α2-抗纤溶酶的纤溶酶原结合型（PB-AP）和非纤溶酶原结合型（NPB-AP）。正常人血浆（HNP）的凝胶过滤显示PB-AP和NPB-AP之间的洗脱体积（KAV）存在差异。PB-AP的KAV为0.33，NPB-AP的KAV为0.39。HNP的等电聚焦显示PB-AP和NPB-AP之间的等电点（pI）存在差异。PB-AP具有异质性，主要峰在pI = 4.69，次要峰在pI = 4.92，而NPB-AP只有一个峰，在pI = 4.59。