等电聚焦管凝胶中血红蛋白的直接分光光度分析
Direct spectrophotometric analysis of hemoglobin in isoelectric focusing tube gels.
作者信息
Magliozzo R S, Park C M, Peisach J
出版信息
Anal Biochem. 1984 Jul;140(1):276-83. doi: 10.1016/0003-2697(84)90165-9.
PMID:6486414
Abstract
Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer. Direct optical sampling of gels allowed the identification of focused hemoglobin valency hybrids which contain two oxidized monomers per tetramer.
摘要
本文描述了对聚焦于聚丙烯酰胺管凝胶中的人氧合血红蛋白、脱氧血红蛋白和高铁血红蛋白进行直接分光光度分析的方法。使用二极管阵列快速扫描分光光度计记录电聚焦血红蛋白条带的可见吸收光谱（350 - 650纳米）。凝胶的直接光学采样能够鉴定出聚焦的血红蛋白价态杂合体，其每个四聚体含有两个氧化单体。
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