Summerfield F W, Tappel A L
Arch Biochem Biophys. 1984 Sep;233(2):408-16. doi: 10.1016/0003-9861(84)90462-4.
Peroxidative damage to DNA was studied in rats fed either a diet with 10% tocopherol-stripped corn oil and 30 IU DL-alpha-tocopherol acetate/kg (group A), the same diet without vitamin E (group B), a diet with 24% corn oil without vitamin E (group C), or the diet of group A for 10 months and then the diet of group C for 4 months (group D). After 3, 6, 9, and 14 months of feeding the diets, body weights, motoric activities, testicular weights, and lipid-soluble fluorophores in testes were measured. Groups A and B had higher hepatic DNA template activities at 9 and 14 months than group C, and group A had higher testicular DNA template activities than groups B and C at 6, 9, and 14 months. Hepatic DNA template activity of group C decreased from 6 to 9 and from 9 to 14 months. Group C hepatic DNA transcribed less long RNA than that of groups B and D, and more short RNA than groups B and D. Group A testicular DNA transcribed more medium-length RNA than that of groups B and D, and less short RNA than that of groups B, C, and D. DNA-bound tryptophan and DNA crosslinking were inversely related to DNA template activities. DNA damage correlated with other biochemical and physiological changes that are characteristic of cellular impairment in aging and disease.
在分别喂食以下几种日粮的大鼠中研究了DNA的过氧化损伤：含10%生育酚去除玉米油和30 IU DL-α-生育酚醋酸酯/千克的日粮（A组）、不含维生素E的相同日粮（B组）、含24%玉米油且不含维生素E的日粮（C组），或A组日粮喂养10个月后再用C组日粮喂养4个月（D组）。在喂食日粮3、6、9和14个月后，测量体重、运动活性、睾丸重量以及睾丸中的脂溶性荧光团。A组和B组在9个月和14个月时的肝脏DNA模板活性高于C组，A组在6、9和14个月时的睾丸DNA模板活性高于B组和C组。C组肝脏DNA模板活性在6至9个月以及9至14个月时下降。C组肝脏DNA转录的长链RNA比B组和D组少，而短链RNA比B组和D组多。A组睾丸DNA转录的中链RNA比B组和D组多，而短链RNA比B组、C组和D组少。与DNA结合的色氨酸和DNA交联与DNA模板活性呈负相关。DNA损伤与衰老和疾病中细胞损伤特征性的其他生化和生理变化相关。
