Umegaki K, Ikegami S, Ichikawa T
National Institute of Health and Nutrition, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1993 Aug;39(4):303-10. doi: 10.3177/jnsv.39.303.
Rats were fed either a low vitamin E (VE) diet (-VE), a basal VE diet (+VE, 4.5 mg%), or a high VE diet (+VE, 45 mg%) for 7 weeks. VE content, oxidative DNA damage and lipid peroxide levels in their livers were measured. When purified lard was used as a dietary fat, VE content decreased in the low VE group to one-thirtieth of that in the basal VE group; in the high VE group it increased to 4.5-fold that in the basal VE group. Corresponding to the VE levels, lipid peroxide levels increased to 2.7-fold in the low VE group and decreased to two-thirds in the high VE group. The level of 8-hydroxydeoxyguanosine (8-OHdG) in DNA, a marker of oxidative DNA damage, was about 0.6 per 10(5) deoxyguanosine in the basal VE group and comparable values were found in the low VE and the high VE groups. When either soybean oil or safflower oil was used as a dietary fat, VE content and lipid peroxide levels in the liver were also markedly changed in both the low VE group and the high VE group. 8-OHdG levels in DNA of the low VE with safflower oil group tended to be higher than that of the basal and high VE groups. However, no significant difference was observed among them. These results suggest that the change in VE has little influence on the level of oxidative DNA damage in the liver.
将大鼠分为三组，分别喂食低维生素E（VE）饮食（-VE）、基础VE饮食（+VE，4.5毫克%）或高VE饮食（+VE，45毫克%），持续7周。测量它们肝脏中的VE含量、氧化性DNA损伤和脂质过氧化物水平。当使用纯化猪油作为膳食脂肪时，低VE组的VE含量降至基础VE组的三十分之一；高VE组则增至基础VE组的4.5倍。与VE水平相对应，低VE组的脂质过氧化物水平增至2.7倍，高VE组则降至三分之二。DNA中氧化性DNA损伤的标志物8-羟基脱氧鸟苷（8-OHdG）水平在基础VE组中约为每10⁵个脱氧鸟苷0.6，在低VE组和高VE组中也有类似数值。当使用大豆油或红花油作为膳食脂肪时，低VE组和高VE组肝脏中的VE含量和脂质过氧化物水平也有明显变化。红花油低VE组DNA中的8-OHdG水平往往高于基础VE组和高VE组。然而，它们之间未观察到显著差异。这些结果表明，VE的变化对肝脏中氧化性DNA损伤水平影响不大。
