心脏病人心内膜心肌活检中辅酶Q10的微量分析以及牛和犬心脏的数据。
Micro-analysis for coenzyme Q10 in endomyocardial biopsies of cardiac patients and data on bovine and canine hearts.
作者信息
Vadhanavikit S, Morishita M, Duff G A, Folkers K
出版信息
Biochem Biophys Res Commun. 1984 Sep 28;123(3):1165-9. doi: 10.1016/s0006-291x(84)80255-7.
A micro-analysis was designed for determining the levels of coenzyme Q10 (CoQ10) in specimens of ca. 500 nanograms of myocardial tissues. The prime steps included acetone extraction, purification by thin-layer chromatography, and HPLC. CoQ11 was the internal standard. This methodology was successful for human endomyocardial biopsies from cardiac patients before and after therapy with CoQ10 and has now been used for canine and bovine tissues. The mean canine CoQ10 level from six specimens from the left ventricle (l.v.) of a single animal is 0.99 +/- 0.06 micrograms/mg/d.w. The mean levels of the tissues from nine animals is 1.04 +/- 0.17 (l.v.) and 1.11 +/- 0.16 (r.v.). The mean bovine level from seven animals was 0.48 +/- 0.12 (l.v.) and 0.68 +/- 0.06 (r.v.).
设计了一种微量分析方法，用于测定约500纳克心肌组织样本中的辅酶Q10（CoQ10）水平。主要步骤包括丙酮萃取、薄层色谱纯化和高效液相色谱法。CoQ11作为内标。该方法成功应用于心脏病患者在接受CoQ10治疗前后的人内心肌活检样本，目前已用于犬类和牛类组织。来自一只动物左心室（l.v.）的六个样本的犬类CoQ10平均水平为0.99±0.06微克/毫克/干重。来自九只动物的组织平均水平为1.04±0.17（左心室）和1.11±0.16（右心室）。来自七只动物的牛类平均水平为0.48±0.12（左心室）和0.68±0.06（右心室）。
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