Temesvári P, Hencz P, Joó F, Eck E, Szerdahelyi P, Boda D
Biol Neonate. 1984;46(4):198-208. doi: 10.1159/000242066.
Acute, bilateral pneumothorax (PT) was produced in 14 newborn piglets. The clinical status of the operated and 14 control animals was monitored by measuring the arterial blood gases, acid-base balance and mean arterial blood pressure. Different brain regions were processed for electron microscopy and albumin immunohistochemistry; water and electrolyte contents were also determined at the end stage of experimental intervention. Electron microscopy showed more intense pinocytotic activity in the endothelium of brain capillaries from PT animals evaluated by morphometry. Statistically significant (p less than 0.01) differences were found in the distribution of pinocytotic vesicles in different brain areas of PT animals. The blood-brain barrier seemed to be impermeable to albumin in all brain regions both in the controls and in the PT group. Parallel with the changes observed in pinocytosis, the water and sodium contents were also increased in the PT group in the parietal cortex (water content 85.18 +/- SD 0.81% vs. 84.10 +/- SD 0.52%, p less than 0.01; sodium content in wet brain tissue 70.94 +/- SD 8.44 mmol/kg vs. 65.09 +/- SD 4.43 mmol/kg, p less than 0.05, in dry brain tissue 481.70 +/- 75.70 mmol/kg vs. 410.15 +/- SD 35.45 mmol/kg, p less than 0.05) and in the cerebellum (water content 83.95 +/- SD 1.08% vs. 83.02 +/- SD 0.89%, p less than 0.05; sodium content in wet brain tissue 60.67 +/- SD 3.16 mmol/kg vs. 55.90 +/- 6.26 mmol/kg, p less than 0.01). However, in other brain regions--especially in the water-shed area--there was no correlation between the changes of pinocytosis and water-electrolyte contents of the tissues. It is suggested that the type of edema developing in this severe cardiovascular/hypoxic collapse is cytotoxic of origin and this fact should be more seriously taken into account in the treatment of the disease.
在14只新生仔猪中制造了急性双侧气胸（PT）。通过测量动脉血气、酸碱平衡和平均动脉血压来监测手术动物和14只对照动物的临床状况。对不同脑区进行电子显微镜检查和白蛋白免疫组织化学检测；在实验干预末期还测定了水和电解质含量。电子显微镜检查显示，通过形态计量学评估，PT动物脑毛细血管内皮细胞的胞饮活性更强。在PT动物的不同脑区，胞饮小泡的分布存在统计学显著差异（p小于0.01）。在对照组和PT组的所有脑区，血脑屏障似乎对白蛋白均不渗透。与胞饮作用的变化平行，PT组顶叶皮质（水含量85.18±标准差0.81%对84.10±标准差0.52%，p小于0.01；湿脑组织中钠含量70.94±标准差8.44 mmol/kg对65.09±标准差4.43 mmol/kg，p小于0.05，干脑组织中481.70±75.70 mmol/kg对410.15±标准差35.45 mmol/kg，p小于0.05）和小脑（水含量83.95±标准差1.08%对83.02±标准差0.89%，p小于0.05；湿脑组织中钠含量60.67±标准差3.16 mmol/kg对55.90±6.26 mmol/kg，p小于0.01）中的水和钠含量也增加。然而，在其他脑区——尤其是分水岭区——胞饮作用的变化与组织的水电解质含量之间没有相关性。提示在这种严重的心血管/缺氧性衰竭中发生的水肿类型起源于细胞毒性，在该疾病的治疗中应更严肃地考虑这一事实。
