Orlacchio A, Maffei C, Beccari T, Lisi P
Clin Chim Acta. 1984 Aug 31;141(2-3):127-33. doi: 10.1016/0009-8981(84)90005-6.
beta-N-Acetyl-D-glucosaminidase and its kinetic characteristics were determined in serum and skin from both diabetes mellitus and impaired glucose tolerance patients and controls. Mean total activity was reduced (p less than 0.05) in the skin of both patient groups. beta-N-Acetyl-D-glucosaminidase isoenzyme expression was investigated using chromatofocusing on PBE-94 coupled with automated enzyme assay. The isoenzyme profiles from serum showed two major forms (A and B) whose ratio varied from 2:1 in controls to 3:1 in diabetics. Moreover, diabetes mellitus and impaired glucose tolerance subjects displayed an intermediate (I) form. The A/B isoenzyme ratio was completely reversed in skin.
在糖尿病患者、糖耐量受损患者及对照组的血清和皮肤中测定了β-N-乙酰-D-氨基葡萄糖苷酶及其动力学特征。两组患者皮肤中的平均总活性均降低（p<0.05）。采用PBE-94柱上聚焦结合自动酶法检测β-N-乙酰-D-氨基葡萄糖苷酶同工酶表达。血清同工酶谱显示有两种主要形式（A和B），其比例在对照组中为2:1，在糖尿病患者中为3:1。此外，糖尿病患者和糖耐量受损受试者还表现出一种中间（I）形式。皮肤中的A/B同工酶比例则完全相反。
