Activity of isoenzyme A and N-acetyl-beta-glucosaminidase in fibrotic liver tissue.

Isoenzymes of N-acetyl-beta-glucosaminidase were separated in the supernatant of 72 homogenized normal and fibrotic human liver. Patterns of isoenzymes were obtained by electrofocusing in thin layers of polyacrylamide gel. The activity of mesenchymal reaction in the liver tissue was determined by histological examination. The distribution of N-acetyl-beta-glucosaminidase isoenzymes was compared with the mesenchymal activity in the liver tissue. The results show a good correlation of isoenzyme A activity with mesenchymal activity.