Musch K, Wolf A S, Lauritzen C
Geburtshilfe Frauenheilkd. 1978 May;38(5):365-70.
The HPL-concentrations of 103 plasma samples from normal and 58 samples from risk pregnancies were analyzed with two radioimmunological assay kits from Pharmacia/Freiburg (I) and Ames/Frankfurt (II). Intra- and inter-assay-variation of both methods are similar. The absolute values of HPL-concentrations analyzed by the two methods correspond to a value of about 3.5 microgram/ml (about week 24 of pregnancy). Above this value higher concentrations are analyzed with method II. These differences probably result from use of different standards and different methods of separation of free and bound hormone. Analysis of variance shows a high degree of correlation (r = 0.93, P less than 0.001, n = 147) of the results obtained by both methods.
使用来自Pharmacia/弗莱堡（I）和Ames/法兰克福（II）的两种放射免疫分析试剂盒，对103份正常孕妇血浆样本和58份高危妊娠孕妇血浆样本的人胎盘催乳素（HPL）浓度进行了分析。两种方法的批内和批间变异相似。两种方法分析的HPL浓度绝对值对应于约3.5微克/毫升的值（约妊娠24周）。高于此值时，用方法II分析得到的浓度更高。这些差异可能是由于使用了不同的标准以及游离和结合激素的不同分离方法。方差分析显示两种方法得到的结果具有高度相关性（r = 0.93，P < 0.001，n = 147）。
