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[用于放射免疫法测定绒毛膜生长催乳激素的5分钟孵育]

[5 minutes incubation for the radioimmunologic determination of somatomammotrophic chorionic hormone].

作者信息

Cesselin F, Antreassian J, Schaffhauser O, Lagoguey M, Desgrez P

出版信息

Ann Biol Clin (Paris). 1976;34(1):41-5.

PMID:970698
Abstract

A radioimmunoassay procedure for human chorionic somatomammotrophin in indiluted plasma has been developed in which the incubation lasts only five minutes rather than one half hour to four days in the previously described methods. The values obtained had excellent correlation (p less than or equal to 0,001) with those obtained by a two-hours technique. The intra-assay and inter-assay coefficients of variation are respectively 7 percent and 11 percent. Twenty women at term showed human chorionic somatomammotrophin values of 7.5 +/- 2.5 mug/ml plasma (mean +/- SD).

摘要

已开发出一种用于检测稀释血浆中人绒毛膜生长催乳素的放射免疫分析方法,该方法的孵育时间仅为5分钟,而不是先前所述方法中的半小时至四天。所获得的值与通过两小时技术获得的值具有极好的相关性(p小于或等于0.001)。批内和批间变异系数分别为7%和11%。20名足月孕妇的血浆中人绒毛膜生长催乳素值为7.5±2.5微克/毫升(平均值±标准差)。

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