Wasilauskas B L, Hampton K D
Diagn Microbiol Infect Dis. 1984 Sep;2(4):301-7. doi: 10.1016/0732-8893(84)90061-0.
Five methods for identification of Streptococcus pneumoniae were evaluated with stock strains representing all 83 capsular types and 130 fresh clinical isolates of alpha-hemolytic streptococci. The identification methods included bile solubility, optochin sensitivity, countercurrent-immunoelectrophoresis (CIEP), and coagglutination (CoA) using laboratory-prepared reagents (LPR) and the Phadebact Pneumococcus Test (Phadebact). The Quellung reaction was performed on the 83 capsular types of S. pneumoniae and on the clinical isolates that produced serological cross reactions with the three serological tests and those that were bile-soluble and optochin-sensitive. All 83 pneumococcal types were in complete agreement with each of the different test methods. Of the 130 alpha-hemolytic streptococci, 26 were identified as S. pneumoniae, and 104 isolates were identified as viridans streptococci using biochemical, physiologic, Quellung, or mouse virulence tests. All 104 viridans streptococci were bile-insoluble and optochin-resistant; however, 63 reacted with either one, both, or all three serological methods. Our data suggest that bile solubility and optochin tests are more reliable for pneumococcal identification than serological methods currently available.
使用代表所有83种荚膜型的标准菌株以及130株α-溶血性链球菌新鲜临床分离株，对5种肺炎链球菌鉴定方法进行了评估。鉴定方法包括胆汁溶解试验、奥普托欣敏感性试验、使用实验室制备试剂（LPR）的对流免疫电泳（CIEP）和协同凝集试验（CoA）以及菲德巴克肺炎球菌试验（Phadebact）。对83种肺炎链球菌荚膜型以及与这三种血清学试验产生血清学交叉反应、胆汁溶解且对奥普托欣敏感的临床分离株进行了荚膜肿胀试验。所有83种肺炎球菌类型与每种不同的试验方法完全一致。在130株α-溶血性链球菌中，使用生化、生理、荚膜肿胀或小鼠毒力试验，26株被鉴定为肺炎链球菌，104株分离株被鉴定为草绿色链球菌。所有104株草绿色链球菌均不溶于胆汁且对奥普托欣耐药；然而，63株与一种、两种或所有三种血清学方法均有反应。我们的数据表明，与目前可用的血清学方法相比，胆汁溶解试验和奥普托欣试验对肺炎球菌的鉴定更可靠。
