Fenoll A, Martinez-Suarez J V, Muñoz R, Casal J, Garcia J L
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
Eur J Clin Microbiol Infect Dis. 1990 Jun;9(6):396-401. doi: 10.1007/BF01979468.
A specific DNA probe containing a 0.65 Kb fragment coding for the aminoterminal region of the major pneumococcal autolysin (amidase) was constructed, deleting the region involved in the specific recognition of cell wall choline residues. The high specificity of this probe was demonstrated in tests with Streptococcus pneumoniae related species including Streptococcus oralis, which contains choline in its cell wall. The probe was used to characterize pneumococcal isolates showing atypical responses in conventional identification tests. The hybridization obtained with 27 atypical pneumococci and 11 of 17 isolates presumptively identified as viridans streptococci confirmed that the probe is suitable for diagnostic use.
构建了一种特异性DNA探针，其包含一个0.65 Kb的片段，该片段编码主要肺炎球菌自溶素（酰胺酶）的氨基末端区域，缺失了参与细胞壁胆碱残基特异性识别的区域。在与肺炎链球菌相关物种（包括口腔链球菌，其细胞壁中含有胆碱）的测试中证明了该探针的高特异性。该探针用于鉴定在传统鉴定试验中表现出非典型反应的肺炎球菌分离株。用27株非典型肺炎球菌和17株推测为草绿色链球菌的分离株中的11株进行杂交，证实该探针适用于诊断用途。
