用菲德巴克凝集试验鉴定肺炎链球菌。
Identification of Streptococcus pneumoniae by the Phadebact coagglutination test.
作者信息
Burdash N M, West M E
出版信息
J Clin Microbiol. 1982 Mar;15(3):391-4. doi: 10.1128/jcm.15.3.391-394.1982.
Abstract
The Phadebact Pneumococcus Test is a coagglutination slide test for the serological identification of Streptococcus pneumoniae. Of 200 alpha-hemolytic streptococcal isolates, coagglutination test results agreed with those of optochin susceptibility and bile solubility in 189 cases, 105 of which were identified as S. pneumoniae by all three methods. The Phadebact test was 100% (113 of 113) sensitive and 98% (85 of 87) specific and was more sensitive than counterimmunoelectrophoresis in detecting the presence of pneumococcal antigen in cerebrospinal fluid. In fluids seeded with known amounts of pneumococcal antigen, it consistently detected lower levels than did counterimmunoelectrophoresis. The test provides a rapid and simple method for the definitive identification of S. pneumoniae.
摘要
法德巴克肺炎球菌试验是一种用于肺炎链球菌血清学鉴定的协同凝集玻片试验。在200株甲型溶血性链球菌分离株中，协同凝集试验结果与奥普托欣敏感性试验和胆汁溶解试验结果在189例中一致，其中105例通过这三种方法均被鉴定为肺炎链球菌。法德巴克试验的敏感性为100%（113/113），特异性为98%（85/87），在检测脑脊液中肺炎球菌抗原的存在方面比对流免疫电泳更敏感。在接种已知量肺炎球菌抗原的液体中，它始终比对流免疫电泳检测到的水平更低。该试验为肺炎链球菌的确切鉴定提供了一种快速简便的方法。
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