Deal E C, Cherniack A D, Eberlin L B
Chest. 1984 Nov;86(5):762-6. doi: 10.1378/chest.86.5.762.
It has been hypothesized that calcium antagonists may be useful in the management of airway hyperreactivity. In these studies, we evaluated the effects of verapamil on guinea pig tracheal spirals and parenchymal lung strips in vitro. Preincubation of both tissues with verapamil caused concentration-dependent inhibition of contraction with significant effects noted at a 10 micromolar concentration. At this concentration of verapamil, approximately fivefold greater concentrations of either histamine or carbachol were required to produce contraction of tracheal spirals; and 21-fold greater concentrations of histamine and 630-fold greater concentrations of carbachol were required to contract parenchymal strips. We also assessed the ability of verapamil to reverse contraction. Significant reversal of both histamine- and carbachol induced contraction was observed with concentrations of 3 micromolar verapamil and contraction was nearly abolished with a 100 micromolar concentration. These data demonstrate that verapamil can both inhibit airway contraction and reverse contraction once it is present and further suggest that verapamil or other calcium antagonists may prove useful in the management of airway hyperreactivity.
据推测，钙拮抗剂可能有助于控制气道高反应性。在这些研究中，我们评估了维拉帕米对豚鼠气管螺旋条和肺实质条的体外作用。用维拉帕米对两种组织进行预孵育会导致收缩的浓度依赖性抑制，在10微摩尔浓度时观察到显著效果。在这个维拉帕米浓度下，产生气管螺旋条收缩所需的组胺或卡巴胆碱浓度大约要高五倍；而使肺实质条收缩所需的组胺浓度要高21倍，卡巴胆碱浓度要高630倍。我们还评估了维拉帕米逆转收缩的能力。观察到3微摩尔浓度的维拉帕米能显著逆转组胺和卡巴胆碱诱导的收缩，而100微摩尔浓度时收缩几乎完全被消除。这些数据表明，维拉帕米既能抑制气道收缩，又能在收缩出现后逆转收缩，进一步表明维拉帕米或其他钙拮抗剂可能被证明对控制气道高反应性有用。
