Drazen J M, Schneider M W
J Clin Invest. 1978 Jun;61(6):1441-7. doi: 10.1172/JCI109063.
The responses of isolated guinea pig tracheal spirals and parenchymal strips to histamine and carbachol were compared. The parenchymal strip, a 1.5 x 1.5 x 20-mm strip cut from the periphery of the lung, constricted at a lower dose and had a larger maximal response to histamine than to carbachol. In contrast, the response of the tracheal spiral to equimolar doses of histamine or carbachol was the same. The responsiveness of both muscle strips to histamine was decreased by treatment with the H1 receptor antagonist mepyramine (0.1 micrometer), and the response to carbachol was blocked by treatment with atropine (0.1 micrometer). Indomethacin (3 micrometer), cimetidine (1 micrometer), propranolol (10 micrometer), and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) (4 micrometer) did not alter the differential response of the two strips to histamine and carbachol. The differential response of parenchymal strips with many, few, or no conducting airways and blood vessels was identical, suggesting that the contractile element is alveolar duct smooth muscle or alveolar contractile elements. This differential pharmacologic response in vitro is consistent with the in vivo observation that histamine causes more peripheral airway constriction than does acetylcholine.
比较了豚鼠离体气管螺旋条和实质条对组胺和卡巴胆碱的反应。实质条是从肺周边切取的1.5×1.5×20毫米的条带，对组胺的收缩反应在较低剂量时出现，且最大反应大于对卡巴胆碱的反应。相比之下，气管螺旋条对等摩尔剂量的组胺或卡巴胆碱的反应相同。用H1受体拮抗剂美吡拉敏（0.1微摩尔）处理后，两条肌肉条对组胺的反应性均降低，用阿托品（0.1微摩尔）处理可阻断对卡巴胆碱的反应。吲哚美辛（3微摩尔）、西咪替丁（1微摩尔）、普萘洛尔（10微摩尔）和N - 乙氧羰基 - 2 - 乙氧基 - 1,2 - 二氢喹啉（EEDQ）（4微摩尔）并未改变两条肌肉条对组胺和卡巴胆碱的差异反应。有许多、少量或无传导气道及血管的实质条的差异反应相同，这表明收缩成分是肺泡管平滑肌或肺泡收缩成分。这种体外的差异药理反应与体内观察结果一致，即组胺比乙酰胆碱引起更多的外周气道收缩。
