Bondok A A, Sansone F M
Exp Neurol. 1984 Nov;86(2):331-41. doi: 10.1016/0014-4886(84)90191-2.
Utilizing recent techniques in quantitative stereology, this investigation studied the synaptology of nucleus dorsalis (Clarke's column) in 12-week-old rats whose sciatic nerves were crushed in the 1st postnatal day. Four morphometric variables were analyzed at the levels of L1 and L3 spinal cord segments: total surface area of synaptic contact zones per unit volume (SV), total length of synaptic contact zones per unit area (LA), average length of synaptic membrane (L), and numerical density of synapses per unit volume (NV). The original raw data were corrected for Holmes's effect. The results indicated that peripheral nerve crush at birth induced a transganglionic change in central sensory terminals with a loss of numerous synapses. A significant loss (P less than 0.001) of about 32% in the SV and LA and a significant loss (P less than 0.001) of about 36% in the NV were observed on the experimental side. There was no preferential loss of synapses in either segment. The mean synaptic membrane length showed no significant difference between the control and experimental sides. The control values of the four morphometric variables calculated for L3 were lower than those calculated for L1. The loss of synapses after a peripheral nerve lesion was probably due to the loss of sensory neurons and their central processes, but there were other possibilities.
利用定量体视学的最新技术，本研究对出生后第1天坐骨神经被挤压的12周龄大鼠背核（克拉克柱）的突触学进行了研究。在L1和L3脊髓节段水平分析了四个形态计量学变量：单位体积突触接触区的总表面积（SV）、单位面积突触接触区的总长度（LA）、突触膜的平均长度（L）和单位体积突触的数量密度（NV）。对原始原始数据进行了霍姆斯效应校正。结果表明，出生时周围神经挤压导致中枢感觉终末发生跨神经节变化，大量突触丧失。在实验侧观察到SV和LA显著减少（P<0.001）约32%，NV显著减少（P<0.001）约36%。两个节段均无突触优先丧失。对照侧和实验侧的平均突触膜长度无显著差异。L3计算的四个形态计量学变量的对照值低于L1计算的值。周围神经损伤后突触的丧失可能是由于感觉神经元及其中枢突的丧失，但也有其他可能性。
