Nichols N R, Khalid B A, Fuller P J, Rayson B M, Funder J W
Mol Cell Endocrinol. 1984 Sep;37(2):197-204. doi: 10.1016/0303-7207(84)90052-2.
We have investigated the domain of adrenal steroid action in a variety of mammalian cells and tissue by high resolution two-dimensional gel electrophoresis and autoradiography. Following in vivo or in vitro treatment with steroids, minced tissue or cells were pulsed with [35S]methionine, and they newly synthesized polypeptides compared with controls by visual inspection of partial protein maps. We have found a similar protein (Mr approximately 41-44K, pI approximately 6.3-6.5) to be consistently increased by dexamethasone in 3 rat tissues and 9 human, bovine and rat cell lines. The protein was constitutively synthesized in all targets; quantitative measurements on the magnitude of response show a 2-fold induction by dexamethasone in all systems, in vivo and in vitro. Glucocorticoid-specific hormones but not sex steroids increase the rate of synthesis; deoxycorticosterone has agonist or antagonist effects on 43K synthesis in different systems studied. Co-electrophoresis experiments indicated that 43K proteins co-migrate in at least 3 rat cell lines from tissues with diverse functions. The functional significance of this common glucocorticoid-induced protein is unknown; its ubiquity and electrophoretic properties suggest a highly conserved, common indicator of glucocorticoid action in diverse target cells and tissues, rather than a tissue-specific response.
我们通过高分辨率二维凝胶电泳和放射自显影技术，研究了肾上腺类固醇在多种哺乳动物细胞和组织中的作用域。在用类固醇进行体内或体外处理后，将切碎的组织或细胞用[35S]甲硫氨酸进行脉冲标记，然后通过目视检查部分蛋白质图谱，将新合成的多肽与对照进行比较。我们发现一种类似的蛋白质（分子量约为41 - 44K，等电点约为6.3 - 6.5）在3种大鼠组织以及9种人、牛和大鼠细胞系中，经地塞米松处理后会持续增加。该蛋白质在所有靶标中均组成性合成；对反应幅度的定量测量表明，在所有体内和体外系统中，地塞米松可诱导其合成增加2倍。糖皮质激素特异性激素而非性类固醇会增加合成速率；在所研究的不同系统中，脱氧皮质酮对43K蛋白的合成具有激动或拮抗作用。共电泳实验表明，43K蛋白在来自具有不同功能组织的至少3种大鼠细胞系中共同迁移。这种常见的糖皮质激素诱导蛋白的功能意义尚不清楚；其普遍性和电泳特性表明，它是不同靶细胞和组织中糖皮质激素作用的高度保守的常见指标，而非组织特异性反应。
