Khalid B A, Gyorki S, Risbridger G P, Warne G L, Funder J W
Mol Cell Endocrinol. 1983 Jun;30(3):303-12. doi: 10.1016/0303-7207(83)90066-7.
Fibroblasts cultured from normal human forearm or genital skin were exposed to dexamethasone (DM) 10(-9)-10(-7) M, deoxycorticosterone (DOC) 10(-6)M, or vehicle for 4-16 h and [35S]methionine-labelled proteins compared by two-dimensional gel electrophoresis. No effects on cell number or [35S]methionine incorporation were seen with either steroid, nor any effects of DOC on patterns of protein synthesis. In contrast, after 4 h incubation DM consistently (24/24 gels) caused increased abundance of a protein spot designated k (kappa; Mr approximately 41 000, pKi approximately 6.5). After 16 h steroid exposure, levels of protein kappa remained elevated; abundance of a second protein spot omega (omega; Mr approximately 42 000, pKi approximately 5.4) was consistently (8/8 gels) lower than in control fibroblasts. We interpret these data as evidence for glucocorticoid-specific effects on human skin fibroblasts, and that (within the pKi and molecular weight range studied) the observed changes in abundance of proteins kappa and omega constitute the human fibroblast glucocorticoid domain.
将从正常人前臂或生殖器皮肤培养的成纤维细胞暴露于10(-9)-10(-7)M的地塞米松（DM）、10(-6)M的脱氧皮质酮（DOC）或溶剂中4-16小时，并通过二维凝胶电泳比较[35S]甲硫氨酸标记的蛋白质。两种类固醇对细胞数量或[35S]甲硫氨酸掺入均无影响，DOC对蛋白质合成模式也无任何影响。相反，孵育4小时后，DM持续（24/24块凝胶）导致一个命名为k（κ；分子量约41000，等电点约6.5）的蛋白质斑点丰度增加。类固醇暴露16小时后，蛋白质κ的水平仍保持升高；另一个蛋白质斑点ω（ω；分子量约42000，等电点约5.4）的丰度始终（8/8块凝胶）低于对照成纤维细胞。我们将这些数据解释为糖皮质激素对人皮肤成纤维细胞有特异性作用的证据，并且（在所研究的等电点和分子量范围内）观察到的蛋白质κ和ω丰度变化构成了人成纤维细胞糖皮质激素结构域。
