Bioulac-Sage P, Lamouliatte H, Saric J, Balabaud C
Gastroenterol Clin Biol. 1984 Aug-Sep;8(8-9):656-9.
Technical details concerning the perfusion-fixation of liver needle biopsies are described. The biopsy (less than or equal to 0,5 cm length) migrated in a Pasteur pipette filled with heparinized Ringer solution and stopped when its diameter corresponded to the internal diameter of the pipette. The pipette was cut above the upper part of the biopsy. The biopsy was perfused with a thin needle (external diameter 0.2 mm) struck in the upper part of the biopsy under binocular microscopy control. Fixation medium, 2.5 p. 100 glutaraldehyde, was aspirated at the inferior extremity of the pipette. The aspiration speed was equal to the perfusion speed (0.15 ml/min). The biopsy was perfused for 5-8 min. This simple technique allows for detailed electron microscopic examination of sinusoids, sinusoidal and perisinusoidal cells as well as of the spaces of Disse.
描述了关于肝穿刺活检组织灌注固定的技术细节。活检组织（长度小于或等于0.5厘米）移入一支装有肝素化林格液的巴斯德吸管中，当活检组织直径与吸管内径一致时停止。吸管在活检组织上部上方切断。在双目显微镜控制下，用一根细针（外径0.2毫米）刺入活检组织上部对其进行灌注。固定液，2.5%的戊二醛，在吸管下端吸出。抽吸速度与灌注速度相等（0.15毫升/分钟）。活检组织灌注5 - 8分钟。这种简单技术可对肝血窦、血窦和血窦周围细胞以及狄氏间隙进行详细的电子显微镜检查。
