Vonnahme F J
Scan Electron Microsc. 1980(3):177-80.
A method for fixing surgical liver biopsies is described. Hepatic tissue is perfused with a needle which is introduced directly into the parenchyma. Fixation is carried out at constant pressure using a perfusion apparatus. The sinusoids of the liver samples, which weigh up to 5g, are flushed completely clean so that the sinusoidal lining cells may be examined by SEM. This technique is particularly suitable for studying the cellular changes which take place in microcirculation disturbances of the human liver.
本文描述了一种用于固定手术肝脏活检组织的方法。用一根直接插入实质的针向肝组织进行灌注。使用灌注装置在恒定压力下进行固定。对重量达5克的肝脏样本的肝血窦进行彻底冲洗，以便能用扫描电子显微镜检查血窦衬里细胞。该技术特别适用于研究人类肝脏微循环障碍中发生的细胞变化。
