Caspi D, Baltz M L, Snel F, Gruys E, Niv D, Batt R M, Munn E A, Buttress N, Pepys M B
Immunology. 1984 Oct;53(2):307-13.
Using calcium-dependent affinity chromatography on Sepharose-bearing, covalently-coupled pneumococcal C-polysaccharide, a protein was isolated from the serum of dogs that had undergone general anaesthesia and major surgery. This protein was confirmed as the canine analogue of C-reactive protein (CRP) in other species by virtue of its electron microscopic appearance, subunit composition and behaviour as an acute phase reactant. Dog CRP had an apparent molecular weight of approximately 100,000 and was composed of five subunits of approximately 20,000 MW each. Two of the five subunits in each molecule were glycosylated. Negatively stained preparations had the typical cyclic pentameric disc-like structure of proteins of the pentraxin family, and in some preparations had a tendency to form stacks. Serum from normal healthy dogs of various strains usually contained less than 5 mg/l of CRP but, following the stimulus of major surgery, an increase in the CRP concentration was first detected at 4 hr.
利用在共价偶联肺炎球菌C多糖的琼脂糖上进行的钙依赖性亲和层析，从经历全身麻醉和大手术的犬血清中分离出一种蛋白质。根据其电子显微镜外观、亚基组成以及作为急性期反应物的行为，该蛋白质被确认为其他物种中C反应蛋白（CRP）的犬类类似物。犬CRP的表观分子量约为100,000，由五个亚基组成，每个亚基的分子量约为20,000。每个分子中的五个亚基中有两个是糖基化的。负染制剂具有五聚体家族蛋白质典型的环状五聚体盘状结构，并且在一些制剂中有形成堆叠的趋势。来自各种品系正常健康犬的血清中CRP通常含量低于5mg/L，但在大手术刺激后，在4小时首次检测到CRP浓度升高。
