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小鼠血清淀粉样蛋白P成分（蛋白质SAP）的分离

Isolation of serum amyloid P-component (protein SAP) in the mouse.

作者信息

Pepys M B

出版信息

Immunology. 1979 Jul;37(3):637-41.

PMID:500121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1457743/
Abstract

Serum amyloid P-component (protein SAP) was associated from mouse serum and ascitic fluids by calcium-dependent affinity chromatography on Sepharose 4B (Pharmacia) followed by gel filtration on Ultrogel AcA44 (LKB) in the presence of EDTA. It was homogenous on gradient polyacrylamide gel and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, and induced a monospecific antiserum in the rabbit. The related protein, C-reactive protein (CRP) was present in only trace amounts not exceeding 2 mug/ml of serum even during acute phase reactions. It was isolated by calcium-dependent affinity chromatography on insolubilized pneumococcal C-polysaccharide. The availability of these isolated mouse proteins and their respective antisera will facilitate investigation of their function.

摘要

血清淀粉样蛋白P成分（蛋白质SAP）通过在琼脂糖4B（Pharmacia）上进行钙依赖性亲和层析，随后在EDTA存在的情况下于Ultrogel AcA44（LKB）上进行凝胶过滤，从小鼠血清和腹水中分离出来。它在梯度聚丙烯酰胺凝胶和十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳上呈均一性，并在兔体内诱导产生单特异性抗血清。相关蛋白C反应蛋白（CRP）即使在急性期反应期间，在血清中的含量也仅为痕量，不超过2微克/毫升。它通过在不溶性肺炎球菌C多糖上进行钙依赖性亲和层析来分离。这些分离出的小鼠蛋白及其各自抗血清的可得性将有助于对其功能的研究。

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本文引用的文献

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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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IgM-producing tumors in the BALB-c mouse: a model for B-cell maturation.BALB-c小鼠中产生IgM的肿瘤：B细胞成熟的模型
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Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.C反应蛋白与补体系统的相互作用。III. 由CRP引发的补体依赖性被动溶血
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No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice.载脂蛋白 E 基因敲除小鼠动脉粥样硬化模型中 CRP 缺乏对动脉粥样硬化形成无影响
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The protective function of human C-reactive protein in mouse models of Streptococcus pneumoniae infection.人C反应蛋白在肺炎链球菌感染小鼠模型中的保护作用。
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Transgenic human C-reactive protein is not proatherogenic in apolipoprotein E-deficient mice.转基因人C反应蛋白在载脂蛋白E缺乏小鼠中无致动脉粥样硬化作用。
Proc Natl Acad Sci U S A. 2005 Jun 7;102(23):8309-14. doi: 10.1073/pnas.0503202102. Epub 2005 May 26.
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