Slepecky N, Larsen H C, Angelborg C
Hear Res. 1984 Aug;15(2):95-101. doi: 10.1016/0378-5955(84)90040-6.
The regional blood flow in the cochlea has been studied by mapping the distribution of microspheres in 22 micron thick serial sections of plastic embedded rat and rabbit cochleas. Using a computer-assisted reconstruction technique, a graphic display of the microspheres in each cochlea is obtained. From this display, microspheres may be counted and their distribution may be observed both in relation to the different vascular areas and with respect to the length of the cochlea. This technique, coupled with quantitation of the microspheres in a reference blood sample, offers a method for calculating total and regional cochlear blood flow using non-radioactive microspheres. In addition, this technique allows analysis of blood flow patterns with respect to the different capillary beds along the length of the cochlea. Thus it may be possible to determine if regional blood flow displays a longitudinal gradient, as do other functional, morphologic and biochemical characteristics of the cochlea.
通过绘制塑料包埋的大鼠和兔耳蜗22微米厚连续切片中微球的分布情况，对耳蜗的局部血流进行了研究。使用计算机辅助重建技术，可获得每个耳蜗中微球的图形显示。通过这种显示，可以对微球进行计数，并观察其相对于不同血管区域以及耳蜗长度的分布情况。该技术与参考血样中微球的定量分析相结合，提供了一种使用非放射性微球计算耳蜗总血流和局部血流的方法。此外，该技术还允许分析沿耳蜗长度不同毛细血管床的血流模式。因此，有可能确定局部血流是否像耳蜗的其他功能、形态和生化特征一样呈现纵向梯度。
