Suckfüll M, Holtmann S, Hecht R
Klinik für Hals-, Nasen- und Ohrenkranke, Ludwig-Maximilians-Universitäts München.
Laryngorhinootologie. 1995 Mar;74(3):141-4. doi: 10.1055/s-2007-997707.
The pathogenesis of sudden hearing loss is as yet unknown. Vascular disturbances are thought to play a major role, but they cannot be diagnosed clinically. We have therefore developed an animal model which allows measurements of the cochlear blood flow (CBF) and function. For CBF measurements a new technique based on coloured microspheres was developed. New Zealand rabbits were anaesthetised, ventilated and blood pressure and heart frequency were continuously monitored. 6 x 10(6) microspheres of one colour were injected in the left ventricle; different colours are available and allow multiple measurements. The amount of microspheres trapped in the capillary bed of the cochlea depends on the regional blood flow. An arterial reference blood sample makes it possible to calculate absolute values of the regional blood flow in the cochlea. The amount of microspheres trapped in the cochlea was determined microscopically after dissecting, dissolving and filtrating the cochlea. Measurements of the CBF in a control group (5 rabbits, 10 inner ears) at the beginning and after 120 minutes were performed. Mean CBF was 3.8 +/- 1.1 microliters/min and 4.3 +/- 1.3 microliters/min. The coloured microsphere technique allows repeated measurements of the CBF with good reproducibility and an acceptable standard deviation. CBF can be measured in absolute values, which is of advantage for determining a lower limit of the CBF for the cochlea function.
突发性听力损失的发病机制尚不清楚。血管紊乱被认为起主要作用，但临床上无法诊断。因此，我们开发了一种动物模型，可用于测量耳蜗血流量（CBF）和功能。为了测量CBF，开发了一种基于彩色微球的新技术。将新西兰兔麻醉、通气，并持续监测血压和心率。将6×10⁶个一种颜色的微球注入左心室；有不同颜色的微球可供使用，可进行多次测量。滞留在耳蜗毛细血管床中的微球数量取决于局部血流量。采集一份动脉参考血样，就可以计算出耳蜗局部血流量的绝对值。在解剖、溶解和过滤耳蜗后，通过显微镜确定滞留在耳蜗中的微球数量。对一个对照组（5只兔子，10只内耳）在开始时和120分钟后进行了CBF测量。平均CBF分别为3.8±1.1微升/分钟和4.3±1.3微升/分钟。彩色微球技术能够对CBF进行重复测量，具有良好的可重复性和可接受的标准差。CBF可以以绝对值进行测量，这对于确定耳蜗功能的CBF下限具有优势。
