Prazma J, Vance S G, Rodgers G
Hear Res. 1984 Apr;14(1):21-8. doi: 10.1016/0378-5955(84)90065-0.
A newly-developed procedure which combines the microsphere method with surface preparation dissection was employed in an investigation of blood flow in cochleas of anesthetized gerbils. In these experiments, 10.1 +/- 0.9 microns carbon-coated microspheres were injected into the left auricle of the heart and a reference blood sample withdrawn at 0.165 ml/min from the iliac artery. The blood flow in the lateral portion (stria vascularis, ligamentum spirale, and suprastria) was calculated to be 337.20, 184.00 and 24.75 nl/min; in the spiral portion (basilar membrane, lamina spiralis ossea, and limbus spiralis) it was found to be 85.40, 39.55 and 6.10 nl/min; and in the central portion (modiolus) it was found to be 256.55, 43.60 and 2.45 nl/min in the first, second and third turns, respectively. The total blood flow in the left and right membranous cochleas was 1062.70 and 896.60 nl/min, respectively. Averaged total blood flow in both ears was 979.60 nl/min. This is a first attempt to measure blood flow in the very small, functionally different areas of the cochlea. This work has shown that it is possible to measure and compare blood flow between the turns and between different functional parts within the turns.
一种将微球法与表面制备解剖相结合的新开发程序被用于研究麻醉沙鼠耳蜗的血流情况。在这些实验中，将直径为10.1±0.9微米的碳包被微球注入心脏的左耳，同时以0.165毫升/分钟的速度从髂动脉抽取参考血样。计算得出外侧部分（血管纹、螺旋韧带和螺旋上区）的血流分别为337.20、184.00和24.75纳升/分钟；螺旋部分（基底膜、螺旋骨板和螺旋缘）的血流分别为85.40、39.55和6.10纳升/分钟；中央部分（蜗轴）在第一、第二和第三圈的血流分别为256.55、43.60和2.45纳升/分钟。左右膜性耳蜗的总血流分别为1062.70和896.60纳升/分钟。双耳的平均总血流为979.60纳升/分钟。这是首次尝试测量耳蜗非常小的、功能不同区域的血流。这项工作表明，有可能测量并比较不同圈之间以及同一圈内不同功能部分之间的血流情况。
