High-pressure size-exclusion chromatography of anticoagulant materials.

A comparative determination of molecular weights of anticoagulant materials is made on different types of high-performance liquid chromatographic stationary phases in aqueous media. The influence of different elution parameters is reported. Narrow-molecular-weight-distribution polystyrenesulphonates are used as standards for the calibration curves. Slight retentions are observed on unmodified silica supports. The commercially available grafted silica columns give similar results for the molecular weight values of heparin. A retention of pentosan polysulphate is observed on a TSK column. However, the size of this anticoagulant polymer is determined on the other grafted silica columns.