Muller D, Ndoume-Nze M, Jozefonvicz J
J Chromatogr. 1984 Aug 3;297:351-8. doi: 10.1016/s0021-9673(01)89055-5.
A comparative determination of molecular weights of anticoagulant materials is made on different types of high-performance liquid chromatographic stationary phases in aqueous media. The influence of different elution parameters is reported. Narrow-molecular-weight-distribution polystyrenesulphonates are used as standards for the calibration curves. Slight retentions are observed on unmodified silica supports. The commercially available grafted silica columns give similar results for the molecular weight values of heparin. A retention of pentosan polysulphate is observed on a TSK column. However, the size of this anticoagulant polymer is determined on the other grafted silica columns.
在水性介质中，利用不同类型的高效液相色谱固定相，对抗凝材料的分子量进行了比较测定。报道了不同洗脱参数的影响。采用窄分子量分布的聚苯乙烯磺酸盐作为校准曲线的标准物。在未改性的硅胶载体上观察到轻微的保留现象。市售的接枝硅胶柱对肝素分子量值给出了相似的结果。在TSK柱上观察到戊聚糖多硫酸盐的保留。然而，这种抗凝血聚合物的尺寸是在其他接枝硅胶柱上测定的。
