de Vries J X
Abteilung für Klinische Pharmakologie, Medizinische Klinik, Universität Heidelberg, F.R.G.
J Chromatogr. 1989 Mar 31;465(2):297-304. doi: 10.1016/s0021-9673(01)92667-6.
High-performance liquid chromatography (HPLC) of heparins was carried out with on-line photodiode-array detection. Average molecular weights and molecular weight distribution were calculated using computer data acquisition and handling; size-exclusion chromatography was performed using different selective microparticulate columns and narrow molecular weight distribution heparin standards for calibration; heparin peak purity was investigated using several methods for evaluation. Additional UV-absorbing compounds present in heparin preparations were characterized and quantitated by reversed-phase HPLC. These methods are useful for the analysis of the molecular weight distribution and peak purity of heparins and for the determination of additional drugs, additives or impurities.
采用在线光电二极管阵列检测法对肝素进行高效液相色谱（HPLC）分析。使用计算机数据采集与处理系统计算平均分子量和分子量分布；采用不同的选择性微粒柱和窄分子量分布肝素标准品进行尺寸排阻色谱法以进行校准；使用多种评估方法研究肝素峰纯度。通过反相HPLC对肝素制剂中存在的其他紫外线吸收化合物进行表征和定量。这些方法对于分析肝素的分子量分布和峰纯度以及测定其他药物、添加剂或杂质很有用。
