Benson J R, Woo D J
J Chromatogr Sci. 1984 Sep;22(9):386-99. doi: 10.1093/chromsci/22.9.386.
Polymeric columns are becoming used more frequently in modern liquid chromatography applications as improvements in polymeric packing materials are realized and as more applications are developed for these materials. Modern polymers have overcome earlier problems associated with their use; higher rigidity now allows them to be used at normal eluent flow rates resulting in faster analyses, and improved synthesis techniques have resulted in efficiencies comparable to the best silica materials, sometimes exceeding 100,000 plates/m. In addition, polymers offer distinct advantages over silica packing for particular applications. Because silica packings are readily degraded by aqueous eluents, they are not always suitable for separations involving ionic species. Polymeric columns are particularly useful in determinations of amino acids, peptides, proteins, organic acids, carbohydrates, and inorganic cations and anions. Polymers are also characterized by exceptional lot-to-lot reproducibility. Reproducibility is often problematic with silica packings, particularly the bonded phases.
随着聚合物填充材料性能的提升以及针对这些材料开发出更多应用，聚合物柱在现代液相色谱应用中越来越频繁地被使用。现代聚合物已经克服了早期使用时出现的问题；如今更高的刚性使它们能够在正常洗脱液流速下使用，从而实现更快的分析，并且改进的合成技术使柱效可与最佳硅胶材料相媲美，有时甚至超过100,000理论塔板数/米。此外，对于特定应用，聚合物相对于硅胶填料具有明显优势。由于硅胶填料容易被水性洗脱液降解，它们并不总是适用于涉及离子物种的分离。聚合物柱在氨基酸、肽、蛋白质、有机酸、碳水化合物以及无机阳离子和阴离子的测定中特别有用。聚合物的另一个特点是批次间具有出色的重现性。硅胶填料，尤其是键合相，批次重现性往往存在问题。
