Kanz L, Löhr G W, Fauser A A
Int J Cell Cloning. 1984 Sep;2(5):286-95.
Medium conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) promotes the growth of multilineage hemopoietic progenitors derived from human bone marrow. However, PHA-LCM prepared in the presence of a human leukocyte interferon preparation does not support mixed colony formation. Crude PHA-LCM preparations were characterized by gel filtration, affinity chromatography, and gel electrophoresis. The elution profile on Sephacryl S-300 of PHA-LCM prepared without interferon showed a distinct peak that stimulated the growth of pluripotent stem cells (CFU-gemm) and committed precursors (CFU-c, BFU-e). Gel filtration of PHA-LCM, prepared with 1000 U/ml of interferon, revealed a change in the elution profile. The eluted material demonstrated no growth-promoting activities. We conclude that the abolished stimulatory activity of PHA-LCM, prepared with human leukocyte interferon, might be due to a reduced production of stimulatory molecules, suggesting that interferon interferes with the molecular events required for colony formation of committed and noncommitted hemopoietic progenitors.
在植物血凝素（PHA-LCM）存在的情况下，由白细胞调节的培养基可促进源自人类骨髓的多谱系造血祖细胞的生长。然而，在人白细胞干扰素制剂存在的情况下制备的PHA-LCM不支持混合集落形成。通过凝胶过滤、亲和色谱和凝胶电泳对粗制PHA-LCM制剂进行了表征。在没有干扰素的情况下制备的PHA-LCM在Sephacryl S-300上的洗脱曲线显示出一个明显的峰，该峰刺激多能干细胞（CFU-gemm）和定向祖细胞（CFU-c、BFU-e）的生长。用1000 U/ml干扰素制备的PHA-LCM的凝胶过滤显示洗脱曲线发生了变化。洗脱的物质没有显示出生长促进活性。我们得出结论，用人白细胞干扰素制备的PHA-LCM的刺激活性丧失可能是由于刺激分子的产生减少，这表明干扰素干扰了定向和未定向造血祖细胞集落形成所需的分子事件。
