Fauser A A, Messner H A
J Supramol Struct Cell Biochem. 1981;15(1):41-8. doi: 10.1002/jsscb.1981.380150105.
Medium conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) promotes growth of human hemopoietic progenitors (CFU-GEMM, BFU-E, CFU-C) and precursors of leukemic blast cells. PHA-LCM was separated by isoelectric focusing and each fraction tested with nonadherent cells of normal individuals as well as blast cells from two patients with acute myelogenous leukemia. Activity profiles for CFU-GEMM, BFU-E and CFU-C ranged form pH 5.0-6.5. The profile for activity stimulatory for leukemic blast cells was broader and ranged from pH 5.5-7.5. Although some overlap was observed, the main peaks of stimulatory activity for normally differentiating progenitors and precursors of leukemic blast cells were separable with respect to their isoelectric point.
在植物血凝素存在的情况下由白细胞产生的条件培养基（PHA-LCM）可促进人类造血祖细胞（CFU-GEMM、BFU-E、CFU-C）以及白血病原始细胞前体的生长。通过等电聚焦分离PHA-LCM，并使用正常个体的非贴壁细胞以及两名急性髓性白血病患者的原始细胞对每个组分进行检测。CFU-GEMM、BFU-E和CFU-C的活性谱范围为pH 5.0 - 6.5。对白血病原始细胞具有刺激活性的谱更宽，范围为pH 5.5 - 7.5。尽管观察到一些重叠，但正常分化祖细胞和白血病原始细胞前体的刺激活性主峰在等电点方面是可分离的。
