Orr E L
J Neurochem. 1984 Nov;43(5):1497-9. doi: 10.1111/j.1471-4159.1984.tb05416.x.
Histamine levels were determined in mouse brains from WBB6F1-+/+ (mast cell normal) and WBB6F1-W/Wv (mast cell-deficient) mice whose brains were dissected immediately after decapitation or after freezing the severed heads in liquid nitrogen for 10 s. In WBB6F1-+/+ mice, brains obtained from frozen heads contained significantly higher levels of histamine than those obtained from unfrozen heads. The converse was found in brains obtained from the WBB6F1-W/Wv mice. When CF-1 mice (which also contain brain-associated mast cells) were treated as described above, results very similar to those found with the WBB6F1-+/+ mice were obtained. Further, the high levels of histamine found in CF-1 mice whose brains had been frozen in situ were accompanied by an extensive degranulation of mast cells in the dura mater of these mice. Because of this degranulation of mast cells, and the fact that increased levels of brain histamine were not found in mast cell-deficient mice, it is concluded that dural mast cells are the likely source of the artifactually higher levels of histamine seen in brains frozen in situ.
测定了WBB6F1-+/+（肥大细胞正常）和WBB6F1-W/Wv（肥大细胞缺陷）小鼠脑内的组胺水平，这些小鼠在断头后或在液氮中将断头冷冻10秒后立即解剖大脑。在WBB6F1-+/+小鼠中，取自冷冻头颅的大脑所含组胺水平显著高于取自未冷冻头颅的大脑。在取自WBB6F1-W/Wv小鼠的大脑中发现了相反的情况。当CF-1小鼠（其也含有与脑相关的肥大细胞）按上述方法处理时，得到的结果与WBB6F1-+/+小鼠的结果非常相似。此外，在大脑原位冷冻的CF-1小鼠中发现的高组胺水平伴随着这些小鼠硬脑膜中肥大细胞的广泛脱颗粒。由于肥大细胞的这种脱颗粒，以及在肥大细胞缺陷小鼠中未发现脑组胺水平升高这一事实，可以得出结论，硬脑膜肥大细胞可能是原位冷冻大脑中出现人为升高的组胺水平的来源。
