Kosztolányi G, Bühler E M
Hum Genet. 1978 May 16;42(1):83-8. doi: 10.1007/BF00291629.
Human lymphoid cell cultures were treated with 2,2'-dithiodipyridine, a thiol reacting agent which produces SS bridges inside the living cells. After 1 to 4 h treatment chromosome preparations were made. The chromosomes acquired a peculiar segmentation along the chromatids. The results suggest that the alteration might arise from a direct reaction of the agent with special chromosomal proteins. The resistance of treated chromosomes to alkali-heat treatment used in C-banding technique was different from that of untreated chromosomes. This prefixational procedure seems to be advantageous in analysing both the chromosome organization and the banding mechanism.
用人淋巴细胞培养物用2,2'-二硫代二吡啶处理，这是一种硫醇反应剂，可在活细胞内产生二硫键桥。处理1至4小时后制备染色体标本。染色体在染色单体上出现了特殊的分段现象。结果表明，这种改变可能源于该试剂与特殊染色体蛋白质的直接反应。处理后的染色体对C带技术中使用的碱热处理的抗性与未处理的染色体不同。这种预处理程序似乎有利于分析染色体组织和显带机制。
