Renlund D G, Gerstenblith G, Lakatta E G, Jacobus W E, Kallman C H, Weisfeldt M L
J Mol Cell Cardiol. 1984 Sep;16(9):795-801. doi: 10.1016/s0022-2828(84)80003-6.
Metabolic and functional recovery following 60 minutes of low flow (0.1 ml/min) ischemia were compared in rabbit hearts perfused with normal sodium and potassium, low sodium (120 mM NaCl replaced by 120 mM LiCl), or zero potassium perfusate during ischemia. During the control, pre-ischemic, and reperfusion periods, all hearts were perfused identically with normal sodium and potassium. 31P NMR was used to monitor intracellular pH (pHi), ATP, and phosphocreatine (PGr). Developed pressure, end diastolic pressure, pHi, and the integrated areas of ATP and PCr were equivalent in the three groups in the pre-ischemic period. The fall in pHi, PCr, ATP, and developed pressure and the rise in end diastolic pressure during 60 min ischemia also did not differ among the three groups. In contrast to the lack of an effect of perfusate sodium and potassium on the decline in parameters of metabolism and function during ischemia, there was a marked difference in the recovery of these indices during reperfusion. Hearts perfused with low sodium during ischemia exhibited the best recovery (expressed as percent of control) of developed pressure (95 +/- 4%), PCr (106 +/- 6%), and ATP (51 +/- 2%) and the smallest rise in end diastolic pressure (229 +/- 50%); hearts perfused with normal sodium and potassium during ischemia had intermediate recovery values for developed pressure (53 +/- 10%), PCr (78 +/- 9%), ATP (45 +/- 4%) and end diastolic pressure (487 +/- 73%) and the hearts perfused with zero potassium solution during ischemia exhibited the poorest recovery of developed pressure (23 +/- 6%), PCr (49 +/- 6%), ATP (39 +/- 5%) and end diastolic pressure (968 +/- 185%).(ABSTRACT TRUNCATED AT 250 WORDS)
对在缺血期间用正常钠钾灌注液、低钠灌注液（用120 mM LiCl替代120 mM NaCl）或零钾灌注液灌注的兔心脏，比较60分钟低流量（0.1 ml/分钟）缺血后的代谢和功能恢复情况。在对照期、缺血前期和再灌注期，所有心脏均用正常钠钾灌注液进行相同方式的灌注。采用31P NMR监测细胞内pH（pHi）、ATP和磷酸肌酸（PCr）。在缺血前期，三组的舒张末压、发展压、pHi以及ATP和PCr的积分面积相当。在60分钟缺血期间，三组的pHi、PCr、ATP下降以及发展压下降和舒张末压上升情况也无差异。与灌注液钠钾对缺血期间代谢和功能参数下降无影响相反，再灌注期间这些指标的恢复存在显著差异。缺血期间用低钠灌注的心脏，发展压（95±4%）、PCr（106±6%）和ATP（51±2%）的恢复情况（以对照百分比表示）最佳，舒张末压上升最小（229±50%）；缺血期间用正常钠钾灌注的心脏，发展压（53±10%）、PCr（78±9%）、ATP（45±4%）和舒张末压（487±73%）的恢复值居中；缺血期间用零钾溶液灌注的心脏，发展压（23±6%）、PCr（49±6%）、ATP（39±5%）和舒张末压（968±185%）的恢复最差。（摘要截短于250词）
