溶酶体促渗性弱碱对辛德毕斯病毒感染BHK - 21细胞的影响。
Effects of lysosomotropic weak bases on infection of BHK-21 cells by Sindbis virus.
作者信息
Cassell S, Edwards J, Brown D T
出版信息
J Virol. 1984 Dec;52(3):857-64. doi: 10.1128/JVI.52.3.857-864.1984.
Abstract
The effects of the lysosomotropic weak bases chloroquine, ammonium chloride, and amantadine as well as dansylcadaverine (an inhibitor of receptor mediated endocytosis) on the replication of Sindbis virus in tissue-cultured cells was examined. Chloroquine had no effect on the expression of virus-induced homologous interference. None of these drugs significantly affected the ability of a complex of a cell and single virion to form an infectious center. Chloroquine and ammonium chloride were found to inhibit the synthesis of virus RNA in established infections when added early in infection. These drugs also inhibited the production of progeny virions when added any time after infection. These results suggest that the antiviral activity of these agents may not be due to an ability to prevent transport of the virus genome into the cell cytoplasm.
摘要
研究了溶酶体促渗弱碱氯喹、氯化铵、金刚烷胺以及丹磺酰尸胺（一种受体介导的内吞作用抑制剂）对辛德毕斯病毒在组织培养细胞中复制的影响。氯喹对病毒诱导的同源干扰的表达没有影响。这些药物均未显著影响细胞与单个病毒粒子复合物形成感染中心的能力。发现氯喹和氯化铵在感染早期添加时可抑制既定感染中病毒RNA的合成。这些药物在感染后的任何时间添加也会抑制子代病毒粒子的产生。这些结果表明，这些药物的抗病毒活性可能并非归因于阻止病毒基因组转运到细胞质中的能力。
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