Goto S, Ikeda K, Nagasaki A, Toyohara T
Jpn J Surg. 1984 Jul;14(4):305-10. doi: 10.1007/BF02469646.
Reliability of the histochemical diagnosis in total colonic aganglionosis is controversial. We studied histochemical acetylcholinesterase (AChE) reactions in three children with total colonic aganglionosis. In all three, there was an increase in AChE positive nerve fibers in the rectal mucosa, obtained when the patients were over the neonatal period. However, a proximal extension of the increase in AChE positive nerve fibers was noted in the distal end of the resected bowel obtained at the definitive operation in two cases out of three. In one, there was no proximal extension in the resected bowel, and in another, there was an increase in AChE positive nerve fibers in the mucosa of the ganglionic ileum. Our findings indicate that the histochemical study of AChE activity in rectal suction biopsy is useful, even in cases of total colonic aganglionosis.
全结肠无神经节症组织化学诊断的可靠性存在争议。我们研究了3例全结肠无神经节症患儿的组织化学乙酰胆碱酯酶（AChE）反应。在所有3例中，当患者度过新生儿期后，直肠黏膜中AChE阳性神经纤维增加。然而，3例中有2例在确定性手术时切除肠段的远端发现AChE阳性神经纤维增加向近端延伸。1例中，切除肠段未见近端延伸，另1例中，神经节性回肠黏膜中AChE阳性神经纤维增加。我们的研究结果表明，即使在全结肠无神经节症病例中，直肠吸引活检中AChE活性的组织化学研究也是有用的。
