用叙利亚仓鼠胚胎细胞进行的非预定DNA合成试验的特性分析。
Characterization of an unscheduled DNA synthesis assay with Syrian hamster embryo cells.
作者信息
Tsutsui T, Suzuki N, Maizumi H, Barrett J C
出版信息
Mutat Res. 1984 Oct;129(1):111-7. doi: 10.1016/0027-5107(84)90129-5.
The Syrian hamster embryo cell transformation assay is widely used for studies of carcinogenesis. The characterization of an unscheduled DNA synthesis (UDS) assay for these cells is reported. Benzo[a]pyrene, aflatoxin B1 and UV light induced UDS in the cells in a dose-dependent manner without exogenous metabolic activity. Nitrosopiperidine induced UDS as well as gene mutations and cell transformation only in the presence of an exogenous metabolic activation system. The utility of this UDS assay with these cells is discussed.
叙利亚仓鼠胚胎细胞转化试验广泛用于致癌作用研究。本文报道了针对这些细胞的非预定DNA合成（UDS）试验的特性。苯并[a]芘、黄曲霉毒素B1和紫外线在无外源代谢活性的情况下以剂量依赖方式诱导细胞中的UDS。亚硝基哌啶仅在存在外源代谢激活系统时诱导UDS以及基因突变和细胞转化。讨论了这种UDS试验在这些细胞中的实用性。
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