Claman H N, Jaffee B D
Transplantation. 1984 Oct;38(4):392-5. doi: 10.1097/00007890-198410000-00015.
The graft-versus-hot (GVH) reaction across minor (non-H-2) histocompatibility barriers was studied in mice, in vivo. To increase GVH potential and to mimic clinical bone marrow transplantation protocols, we modified the popliteal lymph node (PLN) and the splenomegaly assays by irradiating the recipients before they received allogeneic lymphoid cell suspensions. In several combinations across major (H-2), minor (non-H-2) and multiple minor (non-H-2 plus minor lymphocyte stimulation) barriers, increased recipient organ weight (a measure of GVH activity) was seen with irradiated F1 recipients of parental cells. The irradiated splenomegaly (x-splenomegaly) assay was more sensitive than the (x-PLN) assay, but both correlated with in vivo GVH experiments of the P----F1 variety. The x-splenomegaly test indicated histoincompatibility in a system (B10.D2----BALB/c) in which the primary in vitro mixed leukocyte reactions is nonreactive, but in which systemic GVH can be induced. The x-splenomegaly test should be useful in analyzing complex reactions involving minor histocompatibility antigens in vivo.
在小鼠体内研究了跨越次要（非H-2）组织相容性屏障的移植物抗宿主（GVH）反应。为了提高GVH潜能并模拟临床骨髓移植方案，我们在受体接受同种异体淋巴细胞悬液之前对其进行照射，从而改良了腘淋巴结（PLN）和脾肿大检测法。在跨越主要（H-2）、次要（非H-2）和多个次要（非H-2加次要淋巴细胞刺激）屏障的几种组合中，亲代细胞经照射的F1受体出现了受体器官重量增加（一种GVH活性的指标）。照射后的脾肿大（x-脾肿大）检测法比（x-PLN）检测法更敏感，但二者均与P----F1类型的体内GVH实验相关。x-脾肿大检测表明，在一个系统（B10.D2----BALB/c）中存在组织不相容性，在该系统中，原发性体外混合淋巴细胞反应无反应，但可诱导全身性GVH。x-脾肿大检测在分析体内涉及次要组织相容性抗原的复杂反应中应会有用。
