Grebe S C, Streilein J W
Transplantation. 1976 Sep;22(3):245-55. doi: 10.1097/00007890-197609000-00005.
In contrast to the traditional belief that the popliteal lymph node (PLN) assay of graft-versus-host (GVH) reactivity is a local phenomenon, this study describes significant systemic components of both donor and host lymphoid cell activity. Not only are host radiosensitive lymphocytes of systemic origin necessary for the manifestation of normal PLN hypertrophy, but as few as 2.5 X 10(6) parental lymph node cells (LNCs) injected into the hind footpad of adult F1 hybrid rats disseminate widely, provoking significant systemic GVH reactions, as measured by splenomegaly, and distant lymphadenopathy. Futhermore, locally injected donor LNCs ultimately engender three forms of attenuated GVH reactivity: (1) the dissipation of potentially unlimited GVH reactivity, (2) refractoriness of the host to subsequent rechallenge by GVH-inducing cells, and (3) progressive loss of GVH reactivity in donor LNCs when serially transferred to secondary F1 recipients. Whether this modulation of the cell-mediated immune response is the expression of an anti-recognition structure response by the host or the activation of some other immunoregulatory protein, it is absent or reduced in splenectomized recipients. The data suggest that the spleen provides an immunoregulatory microenvironment in which cell-mediated immune responses, such as the GVH reaction, are modulated.
与传统观念认为腘窝淋巴结（PLN）移植物抗宿主（GVH）反应性检测是一种局部现象不同，本研究描述了供体和宿主淋巴细胞活性的重要全身组成部分。正常PLN肥大的表现不仅需要全身来源的宿主放射敏感淋巴细胞，而且向成年F1杂种大鼠后足垫注射低至2.5×10⁶个亲代淋巴结细胞（LNCs）也会广泛播散，引发显著的全身GVH反应，通过脾肿大和远处淋巴结病来衡量。此外，局部注射的供体LNCs最终会产生三种形式的减弱的GVH反应性：（1）潜在无限的GVH反应性的消散，（2）宿主对随后GVH诱导细胞再攻击的难治性，以及（3）当连续转移至二级F1受体时供体LNCs中GVH反应性的逐渐丧失。这种细胞介导免疫反应的调节是宿主抗识别结构反应的表达还是其他免疫调节蛋白的激活，在脾切除的受体中不存在或减弱。数据表明，脾脏提供了一个免疫调节微环境，在其中细胞介导的免疫反应，如GVH反应，受到调节。
