Fritz J C, Pla G W, Harrison B N, Clark G A, Smith E A
J Assoc Off Anal Chem. 1978 May;61(3):709-14.
Variations in the AOAC official first action rat hemoglobin repletion test for iron were studied. These changes included (1) use of a simplified basal diet to eliminate ingredients which sometimes contribute too much iron; (2) increased fortification of the basal diet with vitamin E, pantothenic acid, and pyridoxine; (3) increased dietary copper; (4) variations in the carbohydrate source in the basal diet; (5) changes in the length of the depletion and repletion periods; and (6) comparison of prophylactic and curative procedures. The changes yielded results comparable to those obtained with the present official method. Further study may reveal that the depletion period can be shortened or eliminated. To fully meet the rat's vitamin requirements, increased levels of vitamin E, pantothenic acid, and pyridoxine are recommended. It is further recommended that the present method remain in official first action status, and that study be continued.
对美国官方分析化学家协会（AOAC）铁的大鼠血红蛋白补充试验的各种变化进行了研究。这些变化包括：（1）使用简化的基础日粮以去除有时含铁量过高的成分；（2）增加基础日粮中维生素E、泛酸和吡哆醇的强化量；（3）增加日粮中的铜含量；（4）基础日粮中碳水化合物来源的变化；（5）耗竭期和补充期时长的变化；以及（6）预防性和治疗性程序的比较。这些变化产生的结果与现行官方方法所获得的结果相当。进一步的研究可能表明耗竭期可以缩短或取消。为完全满足大鼠的维生素需求，建议提高维生素E、泛酸和吡哆醇的水平。还建议现行方法保持官方首次采用状态，并继续进行研究。
