Demidova S A, Zakharova L G, Rovnova Z I, Isaeva E I, Poliakova T G
Vopr Virusol. 1984 Jul-Aug;29(4):427-31.
9olid-phase radioimmunoassay (SPRIA) was used for the detection of influenza A (H3N2,H1N1) and B viruses in nasopharyngeal washings of patients admitted in January-March, 1983, to the 1st Clinical Hospital of Moscow City with acute respiratory diseases. The solid phase consisted of nitrocellulose filters and plastic plates which were coated with nasopharyngeal washings of the patients. Rabbit or horse antiviral immunoglobulins were used as antibodies. 125I-labeled protein A was the indicator system. In 61 out of 211 patients examined influenza A (H3N2) virus was detected; from 20 of the influenza A (H3N2), from 7 influenza A (H1N1) virus was isolated, but no influenza B virus was ever found. Comparisons of the results of SPRIA and IF techniques yielded similar but not identical data. Diagnostic rises of antibodies were demonstrated in 48 out of 61 patients. The lack of complete correlation between antibody rises and detection of influenza A virus antigen appears to be due to early discharge of the patients when humoral immunity had not reached its peak. The SPRIA is a highly sensitive and specific technique for influenza A virus detection in nasopharyngeal washings of the patients and may be recommended for use in properly equipped laboratories where highly specific hyperimmune sera are available. It gives an objective information on the proportion of influenza in the period of epidemic rise of ARD incidence.
1983年1月至3月，对莫斯科市第一临床医院收治的患有急性呼吸道疾病的患者，采用固相放射免疫分析（SPRIA）法检测其鼻咽冲洗液中的甲型（H3N2、H1N1）和乙型流感病毒。固相由硝酸纤维素滤膜和塑料板组成，上面包被有患者的鼻咽冲洗液。兔或马抗流感病毒免疫球蛋白用作抗体。125I标记的蛋白A作为指示系统。在211例受检患者中，61例检测到甲型（H3N2）流感病毒；从20例甲型（H3N2）流感患者中分离出7例甲型（H1N1）流感病毒，但未发现乙型流感病毒。SPRIA和免疫荧光（IF）技术结果比较显示数据相似但不完全相同。61例患者中有48例抗体出现诊断性升高。抗体升高与甲型流感病毒抗原检测之间缺乏完全相关性，似乎是由于患者在体液免疫尚未达到峰值时就提前出院。SPRIA是检测患者鼻咽冲洗液中甲型流感病毒的一种高度灵敏且特异的技术，在有高特异性超免疫血清的设备完善的实验室中可推荐使用。它能客观反映在急性呼吸道疾病发病率流行上升期流感所占的比例。
