Isaeva E I, Rovnova Z I, Poliakova T G, Stakhanova V M
Vopr Virusol. 1990 Mar-Apr;35(2):119-21.
In the periods of epidemic increases in the incidence of influenza in 1985-1988, approximately 600 patients with clinical diagnoses of ARVI and influenza were examined for the presence of viral antigen in nasopharyngeal washings by solid-phase enzyme-immunoassay and for antibody rises in paired blood sera. The use of modified SPEIA and original test sera for influenza type A and B viruses in rapid diagnosis of influenza made it possible to decode the etiology of the epidemic situations in 1985-1988. Influenza A and B virus antigens were detected in a high portion of the examined nasopharyngeal washings. The analysis of the distribution of positive results in the detection of viral antigen in the clinical specimens and in influenza serodiagnosis demonstrated a high correlation of the results (93.9%). The etiological pattern of influenza in recent years is characterized by simultaneous circulation in the human population of influenza A (H1N1, H3N2) and B viruses, as reflected by detection of mixed infections in 1-3% of the cases.
在1985 - 1988年流感发病率呈流行上升的时期，通过固相酶免疫测定法对约600例临床诊断为急性呼吸道病毒感染（ARVI）和流感的患者进行了鼻咽冲洗液中病毒抗原检测，并对配对血清中的抗体升高情况进行了检测。使用改良的固相酶免疫测定法（SPEIA）以及针对甲型和乙型流感病毒的原始检测血清进行流感的快速诊断，使得解读1985 - 1988年疫情的病因成为可能。在大部分被检测的鼻咽冲洗液中检测到了甲型和乙型流感病毒抗原。对临床标本中病毒抗原检测结果和流感血清学诊断中阳性结果分布的分析表明，两者结果具有高度相关性（93.9%）。近年来流感的病原学模式表现为甲型（H1N1、H3N2）和乙型流感病毒在人群中同时传播，1 - 3%的病例检测到混合感染即反映了这一点。
