Pierard D, Nusgens B V, Lapiere C M
Anal Biochem. 1984 Aug 15;141(1):127-36. doi: 10.1016/0003-2697(84)90435-4.
Antisera specifically directed against the amino-terminal precursor sequence of bovine p-N-collagen type III (Col 1-3 III) have been raised in rabbit. When tested in a radioimmunoassay, parallel displacement curves and complete cross-reactivity were observed between bovine and human purified Col 1-3 III. However, these displacement curves were not parallel to that obtained with serially diluted human sera which presented a smoother slope. Cleavage of the Col 1-3 III peptide by collagenase at high temperature yielded an immunoreactive preparation containing two smaller peptides (Col 1 plus Col 2) III. The displacement curves obtained with human or bovine (Col 1 plus Col 2) preparations were parallel to those obtained with serially diluted human sera or other human body fluids (amniotic and ascitic fluids). Quantitative measurements of the circulating antigen in normal and pathological conditions are possible only when the (Col 1 plus Col 2) is used as standard antigen. This radioimmunoassay represents an original and simple technique offering good accuracy. The amount of (Col 1 plus Col 2) III in human adults is 69.1 +/- 27.7 ng/ml, with a distribution of frequency close to the normal. It is increased in liver cirrhosis and other conditions characterized by an active neoformation of connective tissue.
已在兔体内制备出特异性针对牛Ⅲ型前胶原氨基末端前体序列（Col 1-3 III）的抗血清。在放射免疫分析中进行检测时，观察到牛和人纯化的Col 1-3 III之间有平行的置换曲线和完全交叉反应性。然而，这些置换曲线与用系列稀释的人血清获得的曲线不平行，后者的斜率更平缓。高温下胶原酶对Col 1-3 III肽的切割产生了一种免疫反应性制剂，其中含有两种较小的肽（Col 1加Col 2）III。用人或牛的（Col 1加Col 2）制剂获得的置换曲线与用系列稀释的人血清或其他人体体液（羊水和腹水）获得的曲线平行。只有当（Col 1加Col 2）用作标准抗原时，才有可能对正常和病理状态下的循环抗原进行定量测量。这种放射免疫分析是一种新颖且简单的技术，具有良好的准确性。人类成年人中（Col 1加Col 2）III的含量为69.1±27.7 ng/ml，频率分布接近正态。在肝硬化和其他以结缔组织活跃新生为特征的疾病中其含量会升高。
