Heynen G, Gaspar S, Gysen P, Plomteux G, Franchimont P
C R Seances Soc Biol Fil. 1981;175(4):533-7.
We have studied the immunoreactivity of human sera and synovial fluids in a radioimmunoassay which utilizes an anti-bovine procollagen type III antiserum and the bovine type III procollagen peptide as standard and tracer. We have noticed a complete cross reaction between bovine col 1-3 and human synovial fluid, which thus contains substance (s) having the same antigenic determinant (s) as col 1-3. On the contrary, we have demonstrated non parallelism between col 1-3 and human serum, whether the serum was obtained from normal subjects or patients suffering from various diseases. These results suggest that immunoreactive substances related to col 1-3 and detected in sera are at least partially different from those detected in synovial fluids.
我们在一项放射免疫测定中研究了人血清和滑液的免疫反应性，该测定使用抗牛III型前胶原抗血清以及牛III型前胶原肽作为标准品和示踪剂。我们注意到牛I-III型胶原与人滑液之间存在完全交叉反应，因此人滑液中含有与I-III型胶原具有相同抗原决定簇的物质。相反，我们已证明I-III型胶原与人血清之间不存在平行关系，无论血清是取自正常受试者还是患有各种疾病的患者。这些结果表明，在血清中检测到的与I-III型胶原相关的免疫反应性物质至少部分不同于在滑液中检测到的物质。
